Variola virus Variola CRMB (aa 23 - 349) [His], recombinant protein from Mouse cells
Recombinant Viral Variola CRMB antigen, was expressed in Mouse myeloma cell line, NS0. Ala23-Leu349, with a C-terminal 6-His tag, Disulfide-linked homodimer (Accession # P34015)
> 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein
2-8°C short term, -20°C long term
Smallpox was an infectious disease unique to humans, caused by either of two virus variants, Variola major and Variola minor. The disease is also known by the Latin names Variola or Variola vera, which is a derivative of the Latin varius, meaning "spotted", or varus, meaning "pimple". The term "smallpox" was first used in Britain in the 15th century to distinguish variola from the "great pox" (syphilis). The last naturally occurring case of smallpox (Variola minor) was diagnosed on 26 October 1977.
Variola virus is the causative agent of human smallpox. Like other poxviruses, it encodes a variety of molecules that shield virus infected cells from immune clearance. The cytokine response modifiers CRMB, C, D, and E, which are differentially expressed among the poxviruses, function as decoy TNF receptors and block the proinflammtatory and antiviral effects of TNF. Of the CRM proteins, Variola virus encodes only CRMB, secreted from virus infected cells as a 90 kDa disulfide linked dimer. The N-terminal 112 amino acid (aa) region of CRMB mediates binding to human, mouse, and rat TNF as well as human lymphotoxin-α, and neutralizes the cytolytic effects of TNF. The C-terminal 155 aa region of CRMB, known as a SECRET domain (smallpox virus-encoded chemokine receptor), binds the chemokines CCL25, CCL28, CXCL12b, CXCL13, and CXCL14, which are involved in the antiviral immune response. Functionally, the SECRETdomain interferes with the in vitro migration of T cells in response to CCL25. A SECRET domain is also present in CRMD but not in CRMC or CRME. Variola virus CRMB shares 84% - 92% aa sequence identity with camelpox virus, cowpox virus, and monkeypox virus CRMB, but only 21% with vaccinia virus CRMB (which lacks a SECRET domain). The TNF binding domain of CRMB shares 30% and 42% aa sequence identity with comparable regions of human TNF R1 and R2, respectively.
Chordopoxvirinae; Poxviridae; Smallpox; Variola; Variola vera; alastrim; cottonpox; milkpox; whitepox; Cuban itch; Variola virus CRMB