Recombinant SARS-CoV Nucleocapsid protein fusioned to His Tag was expressed in E. coli and purified by three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Nature
Recombinant
Tag/Conjugate
His
Alternative Names
SARS Nucleocapsid; SARS; SARS NP; SARS Nucleoprotein; SARS N Protein
Purity
Greater than 95% as determined by SDS-PAGE.
Format
Lyophilized
Buffer
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4.
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length.
Keywords
SARS Nucleocapsid; SARS; SARS NP; SARS Nucleoprotein; SARS N Protein
Citations
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COA
Certificate of Analysis
