RuV [GST], recombinant protein from E. coli
Recombinant rubella capsid protein fused to a GST-Tag at C-terminus was expressed in E. coli and purified by proprietary chromatographic technique.
> 95% pure as determined by 10% PAGE (coomassie staining).
20mM imidazol, 8M urea and 0.3M NaCl.
2-8°C short term, -20°C long term
Rubella virus is an enveloped positive-strand RNA virus of the family TOGAVIRIDAE: Virions are composed of three structural proteins: a capsid and two membrane-spanning glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids.
The capsid protein (CP) has different functions. Its main tasks are the formation of homooligomeres to form the capsid, and the binding of the genomic RNA. Further is it responsible for the aggregation of RNA in the capsid, it interacts with the membrane proteins E1 and E2 and binds the human host-protein p32 which is important for replication of the virus in the host.As opposed to alpha viruses the capsid does not undergo autoprotolysis, rather is it cut off from the rest of the polyprotein by the signal-peptidase. Production of the capsid happens at the surface of intracellular membranes simultaneously with the budding of the virus.
Coat protein; german measles; german measles capsid protein; p110; rubella capsid; Structural polyprotein; Rubella Virus Capsid; Togaviridae; Rubivirus