RSV Active Src [GST], recombinant protein from Baculovirus
Recombinant full-length Rous sarcoma virus Src was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is M11753
The purity was determined to be > 90% by densitometry
2-8°C short term, -20°C long term
Proto-oncogene tyrosine-protein kinase Src is an enzyme that in humans is encoded by the SRC gene. Src is a proto-oncogene encoding a tyrosine kinase originally discovered by J. Michael Bishop and Harold E. Varmus, for which they won the 1989 Nobel Prize in Physiology or Medicine. It belongs to a family of non-receptor tyrosine kinases called Src family kinases. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. This gene is similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
The non-receptor tyrosine kinase Src was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates. The proto-oncogene c-Src is the cellular homologue of viral Src (v-Src) and the founding member of the Src family kinases. c-Src is over-expressed and activated in a large number of human maligancies and has been linked to the development of cancer and progression to distant metastases. In addition to increasing cell proliferation, a key role of c-Src in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumor progression. Although v-Src and c-Src share 88% amino acid identity, v-Src, unlike c-Src, is constitutively active mainly because it lacks a crucial c-terminal negative-regulatory region. As a result, v-Src is missing a tyrosine residue (Y530 in human c-Src) that upon phosphorylation contributes to c-Src assuming an inactive conformation.
c SRC; cSrc; EC 220.127.116.11; Neuronal CSRC tyrosine specific protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000030931; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60-Src; p60Src; pp60c src; pp60c-src; pp60csrc; Proto oncogene tyrosine protein kin