Human U-snRNP B, B′ [His], recombinant protein from Baculovirus
Recombinant humanU-snRNP B/B acute;Full-length cDNA fused to a hexa-histidine purification tag. Expressedby recombinant baculovirus (Autographa californica multiple nuclearpolyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insectcells.
(purity > 80%) SDS-PAGE; Western blot with i: SLE sera (Systemic LupusErythematosus); ii: monoclonal antihexa- His-tag antibody.
0.2–0.4μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Neutral to slightlyalkaline pH; due to purification workup under denaturing conditions presenceof up to 0.02 % SDS (or similar detergents) may be required for maintainingsolubility.
2-8°C short term, -20°C long term
Appears to function in the U7 snRNP complex that is involved in histone 3-end processing. Associated with snRNP U1, U2, U4/U6 and U5. May have a functional role in the pre-mRNA splicing or in snRNP structure. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
Small nuclearribonucleoprotein-associated proteins B and B" is a protein that in humans isencoded by the SNRPB gene. The protein encoded by this gene is one of severalnuclear proteins that are found in common among U1, U2, U4/U6, and U5 smallribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNAsplicing, and the encoded protein may also play a role in pre-mRNA splicingor snRNP structure. Autoantibodies from patients with systemic lupuserythematosus frequently recognize epitopes on the encoded protein. Twotranscript variants encoding different isoforms (B and B") have been foundfor this gene.
SNRPB; small nuclear ribonucleoprotein polypeptides B and B1; SNRPB1; small nuclear ribonucleoprotein-associated proteins B and B; COD; Sm B/B; SmB/SmB; snRNP B; sm-B/Sm-B; Sm protein B/B; B polypeptide of Sm protein; small nuclear ribonucleoprotein polyp