Human Tripartite Motif Containing 21 [His], recombinant protein from Baculovirus
RecombinantFull-length cDNA coding for the human Ro/SS-A (52 kDa) fused to ahexahistidine purification tag. Expressed by recombinant baculovirus (Autographacalifornica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodopterafrugiperda Sf9 inse
SDS-PAGE(purity > 90%); Western blot with i: SLE sera; ii: monoclonal anti-hexa-His-tag antibody.
0.3-0.65μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Neutralto slightly alkaline pH; due to purification workup under denaturingconditions presence of up to 0.02 % SDS (or similar detergents) may berequired for maintaining solubility.
2-8°C short term, -20°C long term
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
Tripartitemotif-containing protein 21 also known as E3 ubiquitin-protein ligaseTRIM21 is a protein that in humans is encoded by the TRIM21 gene.Alternatively spliced transcript variants for this gene have been describedbut the full-length nature of only one has been determined. It is expressedin most human tissues.
TRIM21; tripartite motif containing 21; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS A/Ro) , SSA1,tripartite motif containing 21; E3 ubiquitin-protein ligase TRIM21; RNF81; RO52; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa