Human Thyroid Peroxidase [His], recombinant protein from Baculovirus
Recombinant humanthyroid peroxidase (TPO) was expressed in baculovirus (Autographa californicamultiple nuclear polyhedrosis virus; AcMNPV) infection of Spodopterafrugiperda Sf9 insect cells. Transmembrane andintracytoplasmic parts of the molecule are remo
> 95% (SDS-PAGE);Western blot with i: monoclonal anti-TPO antibody; ii: monoclonalanti-hexa-His-tag antibody
0.15-0.375μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Ionic strengtharound 150 mM, neutral to slightly alkaline pH and 20 % glycerol ascryoprotective agent.
2-8°C short term, -20°C long term
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
Thyroid peroxidaseor thyroperoxidase (TPO) is an enzyme expressed mainly in the thyroid thatliberates iodine for addition onto tyrosine residues on thyroglobulin for theproduction of thyroxine (T4) or triiodothyronine (T3), thyroid hormones. Inhumans, thyroperoxidase is encoded by the TPO gene. Inorganic iodine entersthe body primarily as iodide, I-. After entering the thyroid follicle (orthyroid follicular cell) via a Na+/I- symporter (NIS) on the basolateralside, iodide is shuttled across the apical membrane into the colloid viapendrin, after which thyroid peroxidase oxidizes iodide to atomic iodine (I)or iodinium (I+).
TPO; thyroid peroxidase; MSA; TPX; TDH2A; thyroperoxidase; thyroid microsomal antigen;