Human Thyroid Peroxidase [His] (DAG597)

Human Thyroid Peroxidase [His], recombinant protein from Baculovirus Datasheet

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Product Overview
Recombinant humanthyroid peroxidase (TPO) was expressed in baculovirus (Autographa californicamultiple nuclear polyhedrosis virus; AcMNPV) infection of Spodopterafrugiperda Sf9 insect cells. Transmembrane andintracytoplasmic parts of the molecule are remo
Nature
Recombinant
Tag/Conjugate
His
Purity
> 95% (SDS-PAGE);Western blot with i: monoclonal anti-TPO antibody; ii: monoclonalanti-hexa-His-tag antibody
Concentration
0.15-0.375μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Buffer
Ionic strengtharound 150 mM, neutral to slightly alkaline pH and 20 % glycerol ascryoprotective agent.
Storage
2-8°C short term, -20°C long term
Introduction
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
Antigen Description
Thyroid peroxidaseor thyroperoxidase (TPO) is an enzyme expressed mainly in the thyroid thatliberates iodine for addition onto tyrosine residues on thyroglobulin for theproduction of thyroxine (T4) or triiodothyronine (T3), thyroid hormones. Inhumans, thyroperoxidase is encoded by the TPO gene. Inorganic iodine entersthe body primarily as iodide, I-. After entering the thyroid follicle (orthyroid follicular cell) via a Na+/I- symporter (NIS) on the basolateralside, iodide is shuttled across the apical membrane into the colloid viapendrin, after which thyroid peroxidase oxidizes iodide to atomic iodine (I)or iodinium (I+).
Keywords
TPO; thyroid peroxidase; MSA; TPX; TDH2A; thyroperoxidase; thyroid microsomal antigen;

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