Human SP100 [His], recombinant protein from Baculovirus
RecombinantHumanSP100.Full-length cDNA coding for the human Sp100 protein fused to a hexa-histidinepurification tag. Expressed by recombinant baculovirus (Autographa californicamultiple nuclear polyhedrosis virus; AcMNPV) infection of Spodopterafrugiperda
SDS-PAGE(purity > 80%); Western blot with i: PBC sera; ii: monoclonal anti-hexa-His-tagantibody.
0.5-0.9μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Ionic strength between 150 and 200 mM salt,neutral to slightly alkaline pH and 20% glycerol as cryoprotective agent.
2-8°C short term, -20°C long term
This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]
SP-100 was aU.S. research program for nuclear fission reactors in space. It was startedin 1983 by NASA, the US Department of Energy and other agencies. It developeda reactor with heat pipes transporting the heat to thermionic converters. Itwas cooled with lithium.
SP100; SP100 nuclear antigen; lysp100b; nuclear autoantigen Sp-100; speckled 100 kDa; SP100-HMG nuclear autoantigen; nuclear dot-associated Sp100 protein;