Human Ribosomal Protein [His], recombinant protein from Baculovirus
RecombinantFull-length cDNA coding for the human ribosomal P0 phosphoprotein fused to ahexa-histidine purification tag. Expressed by recombinant baculovirus(Autographa californica multiple nuclear polyhedrosis virus; AcMNPV)infection of Spodoptera frugipe
(purity > 80%) SDS-PAGE; Western blotwith i: SLE sera (Systemic Lupus Erythematosus); ii:monoclonal antihexa- His-tag antibody.
0.3–0.7μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Neutralto slightly alkaline pH; due to purification workup under denaturingconditions presence of up to 0.02 % SDS (or similar detergents) may berequired for maintaining solubility.
2-8°C short term, -20°C long term
Ribosomal protein P0 is the functional equivalent of E.coli protein L10.
60S acidic ribosomalprotein P0 is a protein that in humans is encoded by the RPLP0 gene.Ribosomes, the organelles that catalyze protein synthesis, consist of a small40S subunit and a large 60S subunit. Together these subunits are composed of4 RNA species and approximately 80 structurally distinct proteins. This geneencodes a ribosomal protein that is a component of the 60S subunit. Theprotein, which is the functional equivalent of the E. coli L10 ribosomalprotein, belongs to the L10P family of ribosomal proteins. It is a neutralphosphoprotein with a C-terminal end that is nearly identical to theC-terminal ends of the acidic ribosomal phosphoproteins P1 and P2.
RPLP0; ribosomal protein, large, P0; P0; LP0; L10E; RPP0; PRLP0; 60S acidic ribosomal protein P0; 60S ribosomal protein L10E; acidic ribosomal phosphoprotein P0;