Human Proliferating Cell Nuclear Antigen [His] (DAG615)

Human Proliferating Cell Nuclear Antigen [His], recombinant protein from Baculovirus Datasheet

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Product Overview
Recombinant Human ProliferatingCell Nuclear Antigen, Full-length cDNA coding for the human ProliferatingCell Nuclear Antigen / Cyclin fused to a hexa-histidine purification tag. Expressedbyrecombinant baculovirus (Autographa californica multiplenuclearpol
Nature
Recombinant
Tag/Conjugate
His
Purity
(purity > 90%) SDS-PAGE; Western blot with i: SLE patient sera; ii: monoclonalanti-hexa-His-tag antibody.
Concentration
0.5-1.1μg/ml (depending on the type of ELISA plate and coating buffer; CAUTION:these values are not based on exhaustive testing and are only guidelines forcustomer"s development work). Suitable for biotinylation and iodination.
Buffer
neutral to slightlyalkaline pH, and 20 % glycerol as cryoprotective agent.
Storage
2-8°C short term, -20°C long term
Introduction
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. Many proteins interact with PCNA via the two known PCNA-interacting motifs PCNA-interacting peptide (PIP) box and AlkB homologue 2 PCNA interacting motif (APIM). Proteins binding to PCNA via the PIP-box are mainly involved in DNA replication whereas proteins binding to PCNA via APIM are mainly important in the context of genotoxic stress. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Antigen Description
Proliferating CellNuclear Antigen, commonly known as PCNA, is a protein that acts as aprocessivity factor for DNA polymerase δ in eukaryotic cells. It achievesthis processivity by encircling the DNA, thus creating a topological link tothe genome. It is an example of a DNA clamp. The protein encoded by this geneis found in the nucleus and is a cofactor of DNA polymerase delta. Theencoded protein acts as a homotrimer and helps increase the processivity ofleading strand synthesis during DNA replication. In response to DNA damage,this protein is ubiquitinated and is involved in the RAD6-dependent DNArepair pathway. Two transcript variants encoding the same protein have been foundfor this gene. Pseudogenes of this gene have been described on chromosome 4and on the X chromosome.
Keywords
PCNA; sliding clamp PCNA;

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