Recombinant Papilloma Virus Type 18 antigen, contains the L1 antigen of HPV18, was expressed in Hansenula polymorpha, and puried in vitro using conventional chromatography techniques.
> 90% pure (SDS-PAGE). Purification from broken yeast cells by the following scheme: clarification, ammonium sulfate precipitation, ultrafiltration, cation exchange chromatography, zonal centrifugation, size exclusion chromatography and filtration.
0.75 mg/ml (Lowry)
50mM Sodium phosphate, pH 7.2 containing 200mM Sodium chloride and 0.01% Tween-80
2-8°C short term, -20°C long term
Human papillomavirus (HPV) is a virus from the papillomavirus family that is capable of infecting humans. Like all papillomaviruses, HPVs establish productive infections only in keratinocytes of the skin or mucous membranes. While the majority of the know
L1 is a major capsid protein of type 18 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
Human papillomavirus; HPV;