Human Nucleoporin p62 [His], recombinant protein from Baculovirus
Recombinant Humannucleoporin p62 (Nup62). Full-length cDNA coding for the human nucleoporinp62 fused to a decahistidine purification tag. Expressed by recombinantbaculovirus (Autographa californica multiple nuclear polyhedrosisvirus; AcMNPV) infection of
> 80%(SDS-PAGE). Westernblot with i: polyclonal anti-Nup62 antibodies;ii: monoclonal anti-hexa- His-tag antibody
0.5-1.0μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Ionicstrength around 200 mM salt, neutral to slightly alkaline pH.
2-8°C short term, -20°C long term
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]
Nucleoporin p62(p62) is a protein complex associated with the nuclear envelope. The p62protein remains associated with the nuclear pore complex-lamina fraction. p62is synthesized as a soluble cytoplasmic precursor of 61 kDa followed bymodification that involve addition of N-acetylglucosamine residues. followedby association with other complex proteins.
NUP62; nucleoporin 62kDa; nucleoporin 62kD; nuclear pore glycoprotein p62; DKFZp547L134; FLJ20822; FLJ43869; IBSN; MGC841; p62; SNDI; nucleoporin Nup62; 62 kDa nucleoporin;