Human Nucleoporin [His], recombinant protein from Baculovirus
RecombinantHuman nuclear pore protein gp210. Recombinant construct carrying multiple concatenatedcopies of the short cytoplasmic gp210 C-terminus (which contains theautoreactive epitopes), fused to a hexa-histidine purification tag. Expressedby recombinan
> 80%(SDS-PAGE). Western blot with i: PBC sera; ii:monoclonal anti-hexa-His-tag antibody.
0.4-1.0μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Neutralto slightly alkaline pH and 6 M urea (to maintain denatured state).
2-8°C short term, -20°C long term
Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
Nuclear poreglycoprotein-210 (gp210) is an essential trafficking regulator in theeukaryotic nuclear pore complex. Gp-210 anchors the pore complex to thenuclear membrane. and protein tagging reveals its primarily located on theluminal side of double layer membrane at the pore. A single polypeptide motifof gp210 is responsible for sorting to nuclear membrane, and indicate thecarboxyl tail of the protein is oriented toward the cytoplasmic side of themembrane.
NUP210; nucleoporin 210kDa; GP210; POM210; nuclear pore membrane glycoprotein 210; nucleoporin Nup210; nuclear pore protein gp210; pore membrane protein of 210 kDa; nuclear envelope pore membrane protein POM 210;