Human Ku protein [His], recombinant protein from Baculovirus
RecombinantHuman Ku protein, composed of p70 and p80 subunits (p80 is also called p86), cDNAscoding for full-length chains for the Ku p70 and p80 subunits fused to a hexa-histidinepurification tag. Expressed by recombinant baculovirus (Autographa californ
SDS-PAGE;Western blot with i: anti- Ku autoantibody-positive patient sera; ii:monoclonal anti-hexa-His-tag antibody.
0.2-0.6μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Ionstrength around 200 mM, neutral to slightly alkaline pH and 20 % glycerol ascryoprotective agent.
2-8°C short term, -20°C long term
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
Ku70 is a proteinthat, in humans, is encoded by the XRCC6 gene. Together, Ku70 and Ku80 makeup the Ku heterodimer, which binds to DNA double-strand break ends and isrequired for the non-homologous end joining (NHEJ) pathway of DNA repair. Itis also required for V(D)J recombination, which utilizes the NHEJ pathway topromote antigen diversity in the mammalian immune system.
XRCC6; X-ray repair complementing defective repair in Chinese hamster cells 6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1; X-ray repair cross-complementing protein 6; 5-dRP lyase Ku70; Ku autoantigen, 70kDa; DNA repair protein XRCC6; Ku autoantigen p70 subunit;