Human Histidyl-TRNA Synthetase [His] (DAG603)

Human Histidyl-TRNA Synthetase [His], recombinant protein from Baculovirus Datasheet

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Product Overview
Recombinanthuman histidyl-tRNA synthetase (Jo-1) full-length cDNA coding fused to ahexa-histidine purification tag. Expressed by recombinant baculovirus(Autographa californica multiple nuclear polyhedrosis virus; AcMNPV)infection of Spodoptera frugiperda
Nature
Recombinant
Tag/Conjugate
His
Purity
> 90%(SDS-PAGE).
Concentration
0.7-1.0 μg/ml(depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Buffer
Ionicstrength between 200 and 250 mM salt, neutral to slightly alkaline pH and 20%glycerol as cryoprotective agent.
Storage
2-8°C short term, -20°C long term
Introduction
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.
Antigen Description
Aminoacyl-tRNAsynthetases are a class of enzymes that charge tRNAs with their cognate aminoacids. The protein encoded by this gene is a cytoplasmic enzyme which belongsto the class II family of aminoacyl-tRNA synthetases. The enzyme isresponsible for the synthesis of histidyl-transfer RNA, which is essentialfor the incorporation of histidine into proteins. The gene is located in ahead-to-head orientation with HARSL on chromosome five, where the homologousgenes share a bidirectional promoter. The gene product is a frequent targetof autoantibodies in the human autoimmune diseasepolymyositis/dermatomyositis.
Keywords
HARS; histidyl-tRNA synthetase; histidyl-tRNA synthetase, cytoplasmic; histidine tRNA ligase 1; cytoplasmic; EC 6.1.1.21; FLJ20491; HisRS; histidine translase; Histidine tRNA ligase; Histidyl tRNA synthetase; HRS; Human histidyl tRNA synthetase homolog (H

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