Human Centromere Protein B [His] (DAG600)

Human Centromere Protein B [His], recombinant protein from Baculovirus Datasheet

Online Inquiry Add to basket

Product Overview
RecombinantFull-lengthcDNA coding for the human centromere protein B fused to a hexahistidine purificationtag. Expressed by recombinant baculovirus (Autographa californica multiplenuclear polyhedrosis virus; AcMNPV) infection of Spodopterafrugiperda Sf9 i
Nature
Recombinant
Tag/Conjugate
His
Purity
SDS-PAGE(purity > 90%); Western blot with i: Scleroderma patient sera; ii: monoclonalanti-hexa-His-tag antibody.
Concentration
0.15-0.375μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Buffer
ionic strengtharound 150 mM, neutral to slightly alkaline pH and 20 % glycerol ascryoprotective agent.
Storage
2-8°C short term, -20°C long term
Introduction
This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Antigen Description
Centromere protein Bis a highly conserved protein that facilitates centromere formation. It is aDNA-binding protein that is derived from transposases of the pogo DNAtransposon family. It contains a helix-loop-helix DNA binding motif at theN-terminus, and a dimerization domain at the C-terminus. The DNA bindingdomain recognizes and binds a 17-bp sequence (CENP-B box) in the centromericalpha satellite DNA. This protein is proposed to play an important role inthe assembly of specific centromere structures in interphase nuclei and onmitotic chromosomes. It is also considered a major centromere autoantigenrecognized by sera from patients with anti-centromere antibodies.
Keywords
CENPB; centromere protein B, 80kDa; centromere protein B (80kD); major centromere autoantigen B; CENP B; centromere autoantigen B; Centromere protein B; Major centromere autoantigen B; CENP-B; centromere autoantigen B

Citations


Have you cited DAG600 in a publication? Let us know and earn a reward for your research.

Related Products


Customer Reviews


Write a review, share your experiences with others and get rewarded !

Online Inquiry

Name
Phone *
E-mail Address *
Service & Products Interested *
Project Description
Verification Code * Please input "diagnostics" as verification code.

Online Inquiry

Order Info: Human Centromere Protein B [His]

Online Inquiry
  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!
  15% off your first purchase

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket