Human Centromere Protein A [His], recombinant protein from Baculovirus
Recombinant Human CentromereProtein A.Full-length cDNA coding for the human centromere protein A fused to ahexahistidine purification tag. Expressed by recombinant baculovirus(Autographa californica multiple nuclear polyhedrosis virus; AcMNPV)infection of
SDS-PAGE;Western blot with i: Scleroderma patient sera; ii: monoclonalanti-hexa-His-tag antibody.
0.15-0.4μg/ml (depending on the type of ELISA plate and coating buffer). Suitable forbiotinylation and iodination.
Ionstrength ∼150 mM,neutral to slightly alkaline pH and 6 M urea.
2-8°C short term, -20°C long term
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
Centromere proteinA, also known as CENPA, is a protein which in humans is encoded by the CENPAgene. Centromeres are the differentiated chromosomal domains that specify themitotic behavior of chromosomes. The CENPA gene encodes a centromere proteinwhich contains a histone H3 related histone fold domain that is required fortargeting to the centromere.
CENPA; centromere protein A; Cenp-A; histone H3-like centromeric protein A; centrosomin A; centromere autoantigen A;