Recombinant Herpes Simplex Virus-2 antigen (40.6 kDa), contains the HSV-2 gD immunodominant regions and a GST fusion partner, was expressed in E. coli, and puried in vitro using Metal affinity chromatography techniques.
> 95% pure (10% PAGE (coomassie staining). GS-4B Sepharose-Affinity Purification
25mM Tris-HC1, pH 7.2, 1mM EDTA containing 50% glycerol
2-8°C short term, -20°C long term
Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), also known as Human herpes virus 1 and 2 (HHV-1 and -2), are two members of the herpes virus family, Herpesviridae, that infect humans. Both HSV-1 (which produces most cold sores) and HSV-2 (which produces most genital herpes) are ubiquitous and contagious. They can be spread when an infected person is producing and shedding the virus.
Glycoprotein D(gD) binds specifically to the herpesvirus entry mediator receptor (HVEM), thus providing a strong, fixed attachment to the host cell. These interactions bring the membrane surfaces into mutual proximity and allow for other surface glycoproteins to interact.
Herpesviridae; Alphaherpesvirinae; Simplexvirus; Herpes simplex virus 1; HSV-1; Herpes simplex virus 2; HSV-2; Herpes simplex virus; HSV 1&2; Herpes Simplex Virus Type 1 & 2; HSV1 + HSV2 gD; Envelope glycoprotein D; GD; Glycoprotein D; US6