HIV type 2 Envelope glycoprotein 36 (DAG523)

HIV type 2 Envelope glycoprotein 36, recombinant protein from E. coli Datasheet

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Product Overview
Recombinant Human Immunodeficiency Virus Type 2 Envelope, Gp36, contains the HIV-2 immunodominant region of envelope gp36 (Not full-length gp36), but does not contain a fusion partner. Immunoreactive with HIV-2 positive sera. It was expressed in E. coli,
Nature
Recombinant
Tag/Conjugate
Unconjugated
Molecular Weight
16 kDa
Purity
> 95% pure (10% PAGE coomassie staining). DEAE Sepharose chromatography
Format
Purified, Liquid
Concentration
1 mg/ml
Buffer
50mM Tris HCl, 8M Urea, pH 8.4
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
HIV-1 and HIV-2 appear to package their RNA differently. HIV-1 binds to any appropriate RNA whereas HIV-2 preferentially binds to mRNA which creates the Gag protein itself. This means that HIV-1 is better able to mutate. HIV-2 is transmitted in the same ways as HIV-1: Through exposure to bodily fluids such as blood, semen, tears and vaginal fluids. Immunodeficiency develops more slowly with HIV-2.HIV-2 is less infectious in the early stages of the virus than with HIV-1.The infectiousness of HIV-2 increases as the virus progresses.Major differences include reduced pathogenicity of HIV-2 relative to HIV-1, enhanced immune control of HIV-2 infection and often some degree of CD4-independence. Despite considerable sequence and phenotypic differences between HIV-1 and 2 envelopes, structurally they are quite similar. Both membrane-anchored proteins eventually form the 6-helix bundles from the N-terminal and C-terminal regions of the ectodomain, which is common to many viral and cellular fusion proteins and which seems to drive fusion. HIV-1 gp41 helical regions can form more stable 6-helix bundles than HIV-2 gp41 helical regions however HIV-2 fusion occurs at a lower threshold temperature (25°C), does not require Ca2+ in the medium, is insensitive to treatment of target cells with cytochalasin B, and is not affected by target membrane glycosphingolipid composition.
Antigen Description
The env gp36 ectodomain is highly conserved and elicits a type-specific antibody response. Hence, most licensed diagnostic assays incorporate gp36-derived antigens to detect HIV2 specific antibodies. It is becoming important to differentiate between single infection with either HIV1 or HIV2 and dual infection.
Keywords
Gp36; HIV 2; Human immunodeficiency virus 2; Human Immunodeficiency Virus Type 2; HIV-2 Gp36; Human Immunodeficiency Virus Type 2 Gp36; Retroviridae; Lentivirus

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