HIV type 1 Nef (aa 3 - 190) (Nef Antigen Strain IIIB a.a. 3-190), recombinant protein from E. coli
Recombinant Human Immunodeficiency Virus Type 1 Nef Antigen Strain IIIB (a.a. 3-190). HIV-I nef antigen a.a 3 to a.a 190 of the HIV-I nef region. 20kDa with Beta-galactosidase (114 kDa) fused at the N-terminus. Reactive with human HIV positive serum. It w
> 95% pure (SDS-PAGE)
1 mg/ml (OD280nm)
8M Urea, 20mM Tris-HCl, pH 8.0; 10mM Beta-mercaptoethanol
2-8°C short term, -20°C long term
HIV-1 Nef is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of cell surface receptor proteins such as CD4 and MH4 which are important for AIDS virus infection.
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
3'ORF; ABIN; ABIN1; C terminal core protein; F protein; F-protein; Nef; Negative factor; p27; VAN; HIV-1 Nef