Recombinant HCV type 6 Core Antigen (a.a. 2-120) [GST]

Product Overview

Recombinant HCV-6 core immunodominant region. Contains GST fusion partner, was expressed in E. coli. Immunoreactive with HCV positive sera.

Nature

Recombinant

Tag/Conjugate

GST

Purity

> 95% pure (10% PAGE Coomassie staining). GS-4B Sepharose Affinity Purification

Format

Purified, Liquid

Concentration

1 mg/ml

Buffer

25mM Tris-HCl, 1mM EDTA, 1.5M urea containing 50% glycerol

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.

Keywords

Core protein p19; HCcAg; HCV core antigen; HCV core protein; Hepatitis C Virus core protein;