HCV ORF2 (DAG2366)

HCV ORF2 (aa 452 - 617), recombinant protein from E. coli Datasheet

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Product Overview
The E.Coli derived HEV protein contains the HEV immunodominant regions from ORF2 452-617 a.a.
Nature
Recombinant
Tag/Conjugate
Unconjugated
Purity
> 95%, based on SDS PAGE
Format
Each vial contains 100 μg of lyophilized protein in 25mM Tris-HCl, 1mM EDTA, 0.5M urea and 50% glycerol.
Concentration
N/A
Storage
2-8°C short term, -20°C long term
Introduction
Hepatitis E virus is the infective organism that causes hepatitis E. The viral particles are 27 to 34 nanometers in diameter, are non-enveloped and contain a single-strand of positive-sense RNA that is approximately 7300 bases in length. The virus particle was first visualised in 1983 but was only molecularly cloned in 1990. It was previously classified family Caliciviridae. However, its genome more closely resembles the rubella virus. It is now classified as the only member of the genus Hepevirus, and that genus has not yet been assigned to a family.
Antigen Description
Hepatitis E virus (HEV), the causative agent of viral hepatitis, is a non-enveloped positive-stranded RNA virus with an icosahedral capsid of about 27 to 34 nm in diameter. The viral genome has three open reading frames called ORF1, ORF2, and ORF3. The nonstructural proteins required for virus replication and protein processing are encoded by ORF1, while ORF2 encodes the viral capsid protein, and ORF3 a small protein that regulates the cellular environment. In insect cells, the truncated 56 kDa ORF2 protein can self-assemble to form virus-like particles which possess the same antigenic epitopes as the virion. The N-terminal part of ORF2 is reported to contain an endoplasmic reticulum (ER) translocation signal, and its C-terminal region has an RNA binding site. ORF2's C-terminal region also contains several antigenic sites including a neutralization epitope ranging from residues 458 to 607. Studies investigating humoral responses against HEV showed prominent antibody responses against this and other linear or conformational epitopes of ORF2. Thus, recombinant ORF2 protein has been used as a vaccine candidate. However, very little is known about the host cellular targets of ORF2 protein.
Keywords
HEV; HEV ORF2; ORF2; Hepatitis E Virus ORF2; Hepeviridae; Hepevirus

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