Product Overview
Recombinant HCV Core Antigen containing amino acids 2-192 was expressed in E. coli and purified by proprietary chromatographic technique.
Purity
> 95% pure as determined by 10% PAGE (coomassie staining).
Buffer
20mM Tris-Hcl pH 8, 8M urea and 10mM B-ME.
Storage
2-8°C short term, -20°C long term
Introduction
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10, 000 nucleotides and encodes a single polyprotein of about 3, 000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
Antigen Description
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.
Keywords
HCcAg; Core protein p19; HCV core antigen; HCV core protein; Hepatitis C Virus core protein; HCV-1 Core Ag; Hepatitis C Virus Core Antige, genotype 6a; Flaviviridae; Hepacivirus
Citations
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