HCV Nonstructural Protein 5B [His] (DAG1442)

HCV Nonstructural Protein 5B [His], recombinant protein from E. coli Datasheet

Online Inquiry Add to basket

Product Overview
Recombinant HCV NS5B protein fused to a 6xHis tag at C-terminus was expressed in E. coli and purified by proprietary chromatographic technique.
Nature
Recombinant
Tag/Conjugate
His
Purity
> 95% pure as determined by SDS-PAGE.
Buffer
1.5M urea, 25mM Tris-HCl pH 8.0, 0.2% Triton-X and 50% Glycerol.
Storage
2-8°C short term, -20°C long term
Introduction
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins, and several non-structural proteins necessary for viral replication, of which NS5B is one. NS5B RNA-dependant RNA polymerase is responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication. Hepatitis C virus (HCV) can cause chronic hepatitis, cirrhosis and hepatocellular carcinoma. At present there is no vaccine effective against HCV. Host membrane insertion occurs after processing by the NS3 protease.
Antigen Description
NS5 (non structural protein 5) may play a role in the viral RNA replication of the Hepatitis C Virus. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It"s exact role is not currently known (2008). NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication
Keywords
HCV NS5B; Hepatitis C virus NS5B; Non structural protein 5B; p68; RNA dependent RNA polymerase; RNA directed RNA polymerase;

Citations


Have you cited DAG1442 in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !

Online Inquiry

Name
Phone *
E-mail Address *
Service & Products Interested *
Project Description
Verification Code * Please input "diagnostics" as verification code.

Online Inquiry

Order Info: HCV Nonstructural Protein 5B [His]

Online Inquiry
  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!
  15% off your first purchase

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket