Recombinant HCV NS5B protein fused to a 6xHis tag at C-terminus was expressed in E. coli and purified by proprietary chromatographic technique.
> 95% pure as determined by SDS-PAGE.
1.5M urea, 25mM Tris-HCl pH 8.0, 0.2% Triton-X and 50% Glycerol.
2-8°C short term, -20°C long term
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins, and several non-structural proteins necessary for viral replication, of which NS5B is one. NS5B RNA-dependant RNA polymerase is responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication. Hepatitis C virus (HCV) can cause chronic hepatitis, cirrhosis and hepatocellular carcinoma. At present there is no vaccine effective against HCV. Host membrane insertion occurs after processing by the NS3 protease.
NS5 (non structural protein 5) may play a role in the viral RNA replication of the Hepatitis C Virus. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It"s exact role is not currently known (2008). NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication
HCV NS5B; Hepatitis C virus NS5B; Non structural protein 5B; p68; RNA dependent RNA polymerase; RNA directed RNA polymerase;