HCV type 1a Nonstructural Protein 5, recombinant protein from E. coli
Recombinant HCV NS5 protein containing amino acids 2212-2313 was expressed in E. coli and purified by proprietary chromatographic technique.
> 95% pure as determined by 10% PAGE (coomassie staining).
1.5M urea, 25 mM Tris-HCl, pH-8, 0.2% Triton-X and 50% Glycerol.
2-8°C short term, -20°C long term
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10, 000 nucleotides and encodes a single polyprotein of about 3, 000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
NS5 (non structural protein 5) may play a role in the viral RNA replication of the Hepatitis C Virus. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It"s exact role is not currently known (2008). NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication
HCV NS5; Hepatitis C virus non structural protein 5; Hepatitis C virus nonstructural protein 5; Hepatitis C Virus NS5; HCV NS5 Genotype 2a; Hepatitis C Virus NS5 Genotype 2a