HCV Nonstructural Protein 4A, B (aa 1658 - 1863), recombinant protein from E. coli
HCV NS4 antigen (recombinant) NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein. It is 19kDa and with Beta-galactosidase (114kDa) fused at N-terminus, was expressed in E.coli. Immunoreactive with HCV positive serum.
> 95% pure (SDS-PAGE)
1 mg/ml (OD280nm)
8M urea, 20mM Tris-HCl, pH 8.0, 10mM beta-mercaptoethanol
2-8°C short term, -20°C long term
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.
Hepatitis C virus (HCV) is a small (55-65 nm in size), enveloped, positive sense single strand RNA virus in the family Flaviviridae. The structure of the hepatitis C virus consists of a core of genetic material (RNA), surrounded by an icosahedral protective shell of protein, and further encased in a lipid (fatty) envelope of cellular origin. Two viral envelope glycoproteins, E1 and E2, are embedded in the lipid envelope. Hepatitis C virus has a positive sense RNA genome that consists of a single open reading frame of 9600 nucleoside bases. At the 5" and 3" ends of the RNA are the UTR regions, which are not translated into proteins but are important to translation and replication of the viral RNA. The 5" UTR has a ribosome binding site (IRES - Internal Ribosomal Entry Site) that starts the translation of a 3000 amino acid containing protein that is later cut by cellular and viral proteases into 10 active structural and non-structural smaller proteins. NS4A is a non-structural viral protein that binds to a portion of HCV protease, inhibits HCV replication.
HCV; HCV NS4; Hepatitis C Virus nonstructural antigen 4; Non structural protein 4A; Non structural protein 4B; NS4A; NS4B; Flaviviridae; Hepacivirus; Hepatitis C virus; p27; p8; Hepatitis C Virus NS4