HCV type 1a Nonstructural Protein 3 [His] (DAG520)

HCV type 1a Nonstructural Protein 3 (aa 1192 - 1459) [His], recombinant protein from E. coli Datasheet

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Product Overview
Recombinant Hepatitis C Virus NS-3 c33c, Subtype 1a, contains full-length HCV c33c immunodominant region and 6 histidine fusion sequence. Immunoreactive with HCV positive sera including sera with antibodies to NS3 secondary structure domains. It was expre
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
26 kDa (1192-1459aa)
Purity
> 95% pure (10% PAGE). S-Sepharose and Ceramic Hydroxyapatite and Affinity purification
Format
Purified, Liquid
Concentration
1 mg/ml
Buffer
50mM Sodium phosphate, pH 8.3 containing 10mM DTT
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.
Antigen Description
The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
Keywords
HCV NS3 transactivated protein; NS 3; NS3; NS3P; p70; Serine protease/NTPase/helicase; Hepatitis C Virus NS3; Flaviviridae; Hepacivirus; Hepatitis C virus; HCV NS-3

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