HBV Surface Antigen preS2 (DAG2718)

HBV Surface Antigen preS2, recombinant protein from E. coli Datasheet

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Product Overview
The E. coli derived Recombinant Hepatitis B Surface Antigen preS2 is a single non-glycosylated polypeptide chain containing 55 amino acids & having a molecular weight of 5. 7 kDa.
HBsAg protein was purified by proprietary chromatographic technique. HBsAg protein is > 95% pure as determined by 10% PAGE (coomassie staining).
HBsAg protein was lyophilized from 0. 2μm filtered (1mg/ml) solution in 20mM PB, pH 7. 4 and 50mM NaCl.
2-8°C short term, -20°C long term
Hepatitis B Virus (HBV) infection inducesa disease state characterised by liver damage, inflammation and viralpersistence. Infection also increases the risk of hepatocellular carcinoma.HBV belongs to the Hepadnaviridae family of viruses. Its genome consists ofpartially double stranded circular DNA. The DNA is enclosed in anucleocapsid, or core antigen (HBcAg), which is surrounded by a sphericalenvelope (surface antigen or HBsAg). The core antigen shares its sequenceswith the e antigen (HBeAg) but no cross reactivity between the two proteinshas been observed. The HBV genome also encodes a DNA polymerase that alsoacts as a reverse transcriptase.
Antigen Description
Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, MHBs has a 55 amino acid region called preS2.


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