Recombinant HAV P2C-P3A protein containing amino acids 1392-1521 was expressed in E. coli and purified by proprietary chromatographic technique.
> 90% pure as determined by 10% PAGE (coomassie staining).
10mM CBB, pH9.6, 0.1% SDS and 50% glycerol.
2-8°C short term, -20°C long term
Hepatitis A Virus (HAV) is a 27nm nonenveloped, spherical, positive stranded RNA virus, classified within the genus hepatovirus of the picornavirus family and is among the smallest and structurally simplest of the RNA animal viruses. A single large polyprotein is expressed from a large open reading frame extending through most of the genomic RNA. This polyprotein is subsequently cleaved by a viral protease (3Cpro) to form three (possibly four) capsid proteins and several nonstructural proteins. HAV genomic replication occurs exclusively in the cytoplasm of the infected hepatocyte by a mechanism involving an RNA-dependent RNA polymerase.
picomavindae; hepatovirus; HAV P2C; Hepatitis A Virus P2C; Hepatitis A Virus (HAV) P2C; HAV; Hepatitis A Virus