Human GAD65-1 (DAG4556)

Human GAD65-1, recombinant protein from E. coli Datasheet

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Nature
Recombinant
Expression Host
E.coli
Tag/Conjugate
Unconjugated
Molecular Weight
83KDa
Recommended Usage
Application in indirect ELISA for coating procedure.
Purification
>95% by SDS-PAGE.
Format
Liquid
Size
Can be packed on the basis of your request.
Buffer
10mM PB, 0.01% SDS, pH 7.4.
Storage
Short term: 2-8°C; Long term: -20°C
Warnings
This product is intended for research and manufacturing uses only. It is not a diagnostic device. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. This material has been inactivated, however as with all biological materials, it should be handled as potentially infectious. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations.
Introduction
Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor, In mammals, GAD exists in two isoforms encoded by two different genes - GAD1 and GAD2. These isoforms are GAD67 and GAD65 with molecular weights of 67 and 65 kDa, respectively. GAD1 and GAD2 are expressed in the brain where GABA is used as a neurotransmitter, GAD2 is also expressed in the pancreas.
Keywords
Glutamate decarboxylase; glutamic acid decarboxylase; GAD; GAD65-1; GAD65

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