In 50 mM MES, pH 6.0 (0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, 10% glycerol)
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
Residues 199-203 of zwf encode a NNWDN peptide involved in ChpA(MazF) toxicity that is likely converted to NNWNN by AsnA; a zwf deletion prevents NNWNN activity (Kolodkin-Gal, 2007). A null mutation in the zwf gene encoding glucose 6-phosphate-1-dehydrogenase does not affect the growth rate significantly.
Citations
Publication ()
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