E. coli SlyD (aa 1 - 196), recombinant protein from E. coli
Escherichia coli slyD (NP_755987, 1 a.a. - 196 a.a.) full-length recombinant protein expressed inEscherichia coli .
In 20 mM Tris, pH 7.5
2-8°C short term, -20°C long term
Escherichia coli (/?????r?ki? ?ko?la?/; commonly abbreviated E. coli) is a gram-negative, facultatively anaerobic, rod-shaped bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in their hosts, and are occasionally responsible for product recalls due to food contamination. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and preventing colonization of the intestine with pathogenic bacteria.
slyD belongs to the FKBP-type PPIase family.Required for lysis of phiX174 infected cells. It binds nickel and zinc with high affinity and 1:1 stoichiometry, copper and cobalt with lower affinity. No binding is detectable for ferrous, ferric, magnesium and calcium ions. The activity of slyD is considerably smaller than the one found in other PPIases with the same substrate. The substrate specificity carried out with 'suc-Ala-Xaa-Pro-Phe-4NA', where Xaa is the AA tested, was found to be Phe >Ala >Leu.
FKBP type peptidyl prolyl cis trans isomerase slyD; Histidine rich protein; PPIase; Rotamase; WHP; E. coli slyD; Escherichia coli slyD