E. coli RnhA (aa 1 - 155) [His], recombinant protein from E. coli
rnhA, 1-155 aa E.coli, His-tagged, Recombinant, E.coli
Liquid. In 20mM Tris-HC buffer(pH 8.0) containing 10% glycerol, 2mM DTT
1 mg/ml (determined by Bradford assay)
2-8°C short term, -20°C long term
E. coli is the head of the large bacterial family, Enterobacteriaceae, the enteric bacteria, which are faculatively anaerobic Gram negative rods that live in the intestinal tracts of animals in health and disease. Pili are macromolecular structures that allow binding to a digalactoside receptor in the urinary tract. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors.
cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin; rnhA; ribonuclease HI, degrades RNA of DNA-RNA hybrids; EC 18.104.22.168; RNase HI, degrades RNA of DNA-RNA hybrids; participates in DNA replication; Enterobacteriaceae rnhA; Escherichia rnhA; E. coli rnhA; Esc