E. coli PykF (aa 1 - 470) [His], recombinant protein from E. coli
pykF, 1-470aa E.coli, His-tagged, Recombinant, E.coli
Liquid. In 20mM Tris-HC buffer (pH 8.0) containing 10% glycerol
1 mg/ml (determined by Bradford assay)
2-8°C short term, -20°C long term
Escherichia coli is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans, and are occasionally responsible for product recalls due to food contamination. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and by preventing the establishment of pathogenic bacteria within the intestine
pyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis.
ECK1672; JW1666; pykF; pyruvate kinase I; EC 126.96.36.199; pyruvate kinase I (formerly F), fructose stimulated; Enterobacteriaceae pykF; Escherichia pykF; E. coli pykF; Escherichia coli pykF protein; E. coli pykF protein