Recombinant E. coli Glutathione oxidoreductase (a.a. 1-450) [His]

Name: Recombinant E. coli Glutathione oxidoreductase (a.a. 1-450) [His]
SKU: DAG2565
Rating: 5 (1 reviews)

E. coli Glutathione oxidoreductase (aa 1 - 450) [His], recombinant protein from E. coli

COA

SDS

Datasheet

Summary

Product Overview

gor, 1-450 aa E.coli, His-tagged, Recombinant, E.coli

Nature

Recombinant

Tag/Conjugate

His

Purity

> 90% by SDS-PAGE

Format

Liquid. 20mM Tris-HC buffer (pH8.0) containing 10% glycerol, 0.1M NaCl, 1mM DTT

Concentration

1.0 mg/ml (determined by Bradford assay)

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Escherichia coli (/?????r?ki? ?ko?la?/; commonly abbreviated E. coli) is a gram-negative, facultatively anaerobic, rod-shaped bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in their hosts, and are occasionally responsible for product recalls due to food contamination. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and preventing colonization of the intestine with pathogenic bacteria.

Antigen Description

gor, also known as Glutathione reductase, belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of the protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form. The active site of the protein is a redox-active disulfide bond.

Keywords

ECK3485, gorA, JW3467; gor; glutathione oxidoreductase; EC 1.8.1.7; Enterobacteriaceae gor; Escherichia gor; E. coli gor; Escherichia coli gor protein; E. coli gor protein

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Documents

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References

Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY

Authors: Kalinina, N. A.; Samokhvalova, L. V.; Ulitin, A. B.; Vertiev, Yu. V.; Nesmeyanov, V. A.

Abstract

We describe here the cloning, expression, and production of specific single-chain antibodies (scFv) against the recombinant enterotoxin C1 of Staphylococcus aureus. High-affinity scFv were selected from the phage library of human mini antibodies; afterwards, the cells of E. coli trxA gor double mutant were infected with a product obtained by fusion of DNA encoding of these mini antibodies with the trxA gene to induce soluble scFv synthesis in cell cytoplasm. The scFv obtained displayed high enterotoxin C1 affinity. Analysis for cross reactivity showed that mini antibodies interacted also with SEA- SEB-, SED-, SEE-, SEG-, and SEI-type enterotoxins, but they failed to interact with ricin, diphtheritic, and cholera toxins, or with both lethal and protective factors of the anthrax toxin. This property may be helpful in screening for staphylococcus enterotoxins.

Generation of maize cell lines containing autonomously replicating maize streak virus-based gene vectors

ARCHIVES OF VIROLOGY

Authors: Palmer, KE; Thomson, JA; Rybicki, EP

Abstract

Virion sense gene replacement derivatives of maize streak virus (MSV) were constructed with selectable marker expression cassettes based on the bialaphos resistance gene (bar) and the CaMV 35S promoter. The effect on replication of increasing the genomic size was tested by including: (1) the 550-bp maize adh I intron and 68-bp TMV Omega RNA leader sequences upstream of the bar genes; and (2) a fusion between the bar and E. coli glutathione reductase (gor) genes. Three recombinant viral vectors ranging in size from 2.7 kb to 4.8 kb replicated efficiently in biolistically transfected cells of suspension cultured Black Mexican sweetcorn (BMS) cells. Deletions greater than 39 bp 3' of the stemloop sequence in the LIR adversely affected replicon release. Transformed bialaphos-resistant EMS cell lines were generated with all three vectors containing the bar gene: between 38 and 60% of cell lines contained replicating viral episomes. The replicons were structurally stable, replicated to copy numbers of over 500 per haploid genome, and were detected for more than one year after introduction. We noted significant enhancement of bar gene expression, both at the protein and RNA levels, associated with the presence of episomal vector DNA. The maize adhI intron and TMV Omega RNA leader sequences did not seem to have a significant effect on bar gene expression from replicating constructs, although expression from controls was enhanced. The results suggest that MSV-based constructs would provide a useful system for long-term gene amplification in cereal cell culture systems.