E. coli Α-Galactosidase, α-Gal (aa 2 - 708) [His], recombinant protein from E. coli
Recombinant E. coli α-Galactosidase/α-Gal antigen, was expressed in E. coli. Val2-Val708. with an N-terminal Met and 6-His tag (Accession # YP_001464379)
> 95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie. Blue stain at 5 μg per lane.
2-8°C short term, -20°C long term
Escherichia coli is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans, and are occasionally responsible for product recalls due to food contamination. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and by preventing the establishment of pathogenic bacteria within the intestine
α-Galactoside is prevalent in animals and plants. The α-Galactosidase (α-Gal) from Escherichia coli is a useful tool for the removal of α1, 3-linked and α1, 6-linked galactosides from the non-reducing terminus of complex carbohydrates and glycoproteins. α-Gal efficiently hydrolyzes raffinose, an α-D-galactosylsucrose, to D-galactose and sucrose. The enzyme can also hydrolyze other α-Galactosides such as melibiose, stachyose, verbascose, and galactinol. The enzyme does not cleave β-linked galactose, such as lactose.
Agalsidase alfa; Alpha D galactosidase A; Alpha D galactoside galactohydrolase; Melibiase; AGAL; Alpha galactosidase A; Alpha-D-galactosidase A; Alpha-D-galactoside galactohydrolase; Alpha-galactosidase A; GALA; GLA; GLA protein; E. coli α-Gal protein; Es