Recombinant Dengue Type-2 Envelope Domain III (amino acids 300-400) immunodominant regions. It was expressed in E.coli. This region contains neutralizing epitopes, and receptor binding domain. Reacts with Dengue Type 2 IgG. Contains a 6-His fusion partner
> 95% pure (12% PAGE (Coomassie)). Proprietary chromatographic technique.
2-8°C short term, -20°C long term
Dengue virus (DENV) in one of four serotypes is the cause of dengue fever. It is a mosquito-borne single positive-stranded RNA virus of the family Flaviviridae; genus Flavivirus. All four serotypes can cause the full spectrum of disease. Its genome is about 11000 bases that codes for three structural proteins, capsid protein C, membrane protein M, envelope protein E; seven nonstructural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5; and short non-coding regions on both the 5' and 3' ends. Further classification of each serotype into genotypes often relates to the region where particular strains are commonly found or were first found.
Envelope protein E binds cell surface receptor and is involved in membrane fusion between virion and target cell. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociates from small envelope protein M and homodimerizes.
DENV; Dengue Virus Envelope Protein; DENV Envelope Protein; Dengue virus