DENV type 4 Nonstructural Protein 1 (DAG3065)

DENV type 4 Nonstructural Protein 1 (aa 776-1130), recombinant protein from Baculovirus

Product Overview
Recombinant Dengue Type 4 NS1 Protein
Nature
Recombinant
Tag/Conjugate
Unconjugated
Procedure
5 mM EDTA
Purity
≥ 95% on 12.5% SDS-PAGE
Concentration
≥ 1 mg/ml by Bradford dye assay
Buffer
1X PBS, pH7.4
Preservative
0.1% Thimerosal
Storage
2-8°C short term, -20°C long term
Introduction
NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
Keywords
Dengue NS1; Dengue Virus non-structural protein 1; Dengue Virus NS1 glycoprotein; DENV NS1

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References


Quantification of dengue virus specific T cell responses and correlation with viral load and clinical disease severity in acute dengue infection

PLOS NEGLECTED TROPICAL DISEASES

Authors: Wijeratne, Dulharie T.; Fernando, Samitha; Gomes, Laksiri; Jeewandara, Chandima; Ginneliya, Anushka; Samarasekara, Supun; Wijewickrama, Ananda; Hardman, Clare S.; Ogg, Graham S.; Malavige, Gathsaurie Neelika

Background In order to understand the role of dengue virus (DENV) specific T cell responses that associate with protection, we studied their frequency and phenotype in relation to clinical disease severity and resolution of viraemia in a large cohort of patients with varying severity of acute dengue infection. Methodology/Principal findings Using ex vivo IFN gamma ELISpot assays we determined the frequency of dengue viral peptide (DENV)-NS3, NS1 and NS5 responsive T cells in 74 adult patients with acute dengue infection and examined the association of responsive T cell frequency with the extent of viraemia and clinical disease severity. We found that total DENV-specific and DENV-NS3-specific T cell responses, were higher in patients with dengue fever (DF), when compared to those with dengue haemorrhagic fever (DHF). In addition, those with DF had significantly higher (p = 0.02) DENV-specific T cell responses on day 4 of infection compared to those who subsequently developed DHF. DENV peptide specific T cell responses inversely correlated with the degree of viraemia, which was most significant for DENV-NS3 specific T cell responses (Spearman's r = -0.47, p = 0.0003). The frequency of T cell responses to NS1, NS5 and pooled DENV peptides, correlated with the degree of thrombocytopenia but had no association with levels of liver transaminases. In contrast, total DENV-IgG inversely correlated with the degree of thrombocytopenia and levels of liver transaminases. Conclusions/Significance Early appearance of DENV-specific T cell IFN gamma responses before the onset of plasma leakage, appears to associate with milder clinical disease and resolution of viraemia, suggesting a protective role in acute dengue infection.

Immune evasion strategies of flaviviruses

VACCINE

Authors: Ye, Jing; Zhu, Bibo; Fu, Zhen F.; Chen, Huanchun; Cao, Shengbo

Flavivirus is a genus of the family Flaviviridae. It includes West Nile virus (WNV), dengue virus (DENV), yellow fever virus (YFV), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBEV), and several other viruses which lead to extensive morbidity and mortality in humans. To establish infection and replication in the hosts, flaviviruses have evolved a variety of strategies to modulate the host's immune responses. In this review, the strategies employed by flaviviruses to evade the innate and adaptive immunity of host are summarized based on current studies, with a major focus on the inhibition of interferon, complement, natural killer (NK) cell, B cell, and T cell responses. This review aims to provide an overview of the current understanding for the mechanisms used by flaviviruses to escape the host's immune response, which will facilitate the future studies on flavivirus pathogenesis and the development of anti-flavivirus therapeutics. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.

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