Borrelia OspA [His], recombinant protein from E. coli
Recombinant Borrelia afzelii OspA antigen. Expressed in E. coli bacterial cells. Full-length cDNA coding for Borrelia afzelii OspA fused to a hexa-histidine purification tag.
neutral to slightly alkaline pH and 20 % glycerol as cryoprotective agent.
2-8°C short term, -20°C long term
Borrelia afzelii is a species of Borrelia that can infect various species of vertebrate and invertebrates. Among thirty Borrelia known species, it is one of 4 which is likely to infect humans causing a variant of Lyme disease. It seems fairly common that coinfection combine this Borrelia specie to another one or to other pathogens carried by the vector, which appears to be in most cases the tick.
Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.
Bacteria; Spirochaetes; Spirochaetales; Spirochaetaceae; Borrelia; Borrelia garinii; Borrelia burgdorferi (Lyme); Borrelia burgdorferi; Lyme; Borrelia burgdorferi garini; B. afzelii OspA protein; Borrelia afzelii outer surface protein A; OspA; outer surfa