A. caninum NIF [His] (DAG2624)

A. caninum NIF (aa 18 - 274) [His], recombinant protein from Mouse cells

Product Overview
Recombinant A. caninum NIF antigen, was expressed in Mouse myeloma cell line, NS0. Asn18-Leu274, with a C-terminal 6-His tag (Accession # AAA27789)
Nature
Recombinant
Tag/Conjugate
His
Procedure
None
Purity
> 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Format
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, and DTT
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
Ancylostoma caninum, commonly Dog hookworm, is a parasitic nematode hookworm that infects dogs. The larval stage penetrates the skin and makes it way through the circulatory system into the digestive tract, where adult forms lay eggs that are passed through the feces. Common symptoms include anemia and diarrhea. Newborn pups can die of hemorrhaging from their intestines caused by massive numbers of feeding hookworms. A. braziliense and Uncinaria stenocephala are different species of hookworm which can infect dogs and cause similar symptoms
Antigen Description
Neutrophil inhibitory factor (NIF) of the dog hookworm, Ancylostoma caninum, is a 41 kDa secreted glycoprotein that is thought to allow the hookworm to evade the innate immune defenses of the host. NIF interacts with the leukocyte integrin αMβ2 (CD11b/CD18, also called Mac-1), thus inhibiting mammalian neutrophil adhesion to endothelium. The A. caninum NIF cDNA encodes 257 amino acids (aa) including a 17 aa signal sequence and a 240 aa mature protein with 10 cysteine residues and 7 potential N-linked glycosylation sites. Incubation of mammalian neutrophils with NIF does not appear to be toxic, but does result in rapid, cation-dependent, reversible inhibition of αMβ2-mediated adhesion and degranulation. The NIF binding site within the αMβ2 I-domain interferes with activation-dependent adhesion sites of ligands including ICAM-1/CD54, complement component C3b, and fibrinogen. In mouse or guinea pig inflammatory lung injury models, administration of NIF prevented neutrophil-dependent vascular injury.
Keywords
Ancylostoma caninum; A. caninum; caninum; A. caninum NIF; Neutrophil inhibitory factor; Ancylostoma caninum Neutrophil inhibitory factor protein

Citations


Have you cited DAG2624 in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

Online Inquiry

Name:
Phone: *
E-mail Address: *
Service & Products Interested: *
Project Description:
Verification Code: * Please input "diagnostics" as verification code.

Online Inquiry

  Interested in larger quantities ? request a quote!
  Protocol may be improved. Please feel free to contact us to obtain the latest version.!

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

OUR PROMISE TO YOU Guaranteed product quality expert customer support

Inquiry Basket