Rat Immunoglobulin Isotyping ELISA Kit (DEIA6802)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
hybridoma culture supernatant
Species Reactivity
Rat
Intended Use
The Rat Immunoglobulin Isotyping ELISA Kit enables rapid, efficient identification of rat immunoglobulin isotypes.
Contents of Kit
1. Mouse anti-rat IgG1 purfied mAb*: 0.25 ml (working dilution: 1:50)
2. Mouse anti-rat IgG2a purfied mAb*: 0.25 ml(working dilution: 1:50)
3. Mouse anti-rat IgG2b purfied mAb*: 0.25 ml(working dilution: 1:50)
4. Mouse anti-rat IgG2c purfied mAb*: 0.25 ml(working dilution: 1:50)
5. Mouse anti-rat IgM purfied mAb*: 0.25 ml (working dilution: 1:50)
6. Mouse anti-rat IgA purfied mAb*: 0.25 ml (working dilution: 1:50)
7. Mouse anti-rat Igκ purfied mAb*: 0.25 ml (working dilution: 1:50)
8. Mouse anti-rat Igλ purfied mAb*: 0.25 ml (working dilution: 1:50)
9. HRP-labeled mouse anti-rat Ab+: 1.0 ml (working dilution: 1:100)
10. Substrate Reagent A: 40 ml
11. Substrate Reagent B: 40 ml
12. Stop Solution: 40 ml
13. Positive Reference Antigen Mixture*: 0.25 ml (working dilution: 1:50)
14. 10×PBS Buffer: 40 ml (working dilution: 1:10)
15. 10% BSA** Solution+: 30 ml (working dilution: 1:10)
Storage
Each monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The mouse anti-rat Ig antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed. All components of the Kit should be store undiluted at 4°C. Do not freeze.
General Description
Immunoglobulin (Ig), is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target, called an antigen. Each tip of the "Y" of an antibody contains a paratope (a structure analogous to a lock) that is specific for one particular epitope (similarly analogous to a key) on an antigen, allowing these two structures to bind together with precision. Using this binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize its target directly (for example, by blocking a part of a microbe that is essential for its invasion and survival). The production of antibodies is the main function of the humoral immune system.

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