96T

hybridoma culture supernatant

Rat

The Rat Immunoglobulin Isotyping ELISA Kit enables rapid, efficient identification of rat immunoglobulin isotypes.

1. Mouse anti-rat IgG1 purfied mAb*: 0.25 ml (working dilution: 1:50)
2. Mouse anti-rat IgG2a purfied mAb*: 0.25 ml(working dilution: 1:50)
3. Mouse anti-rat IgG2b purfied mAb*: 0.25 ml(working dilution: 1:50)
4. Mouse anti-rat IgG2c purfied mAb*: 0.25 ml(working dilution: 1:50)
5. Mouse anti-rat IgM purfied mAb*: 0.25 ml (working dilution: 1:50)
6. Mouse anti-rat IgA purfied mAb*: 0.25 ml (working dilution: 1:50)
7. Mouse anti-rat Igκ purfied mAb*: 0.25 ml (working dilution: 1:50)
8. Mouse anti-rat Igλ purfied mAb*: 0.25 ml (working dilution: 1:50)
9. HRP-labeled mouse anti-rat Ab+: 1.0 ml (working dilution: 1:100)
10. Substrate Reagent A: 40 ml
11. Substrate Reagent B: 40 ml
12. Stop Solution: 40 ml
13. Positive Reference Antigen Mixture*: 0.25 ml (working dilution: 1:50)
14. 10×PBS Buffer: 40 ml (working dilution: 1:10)
15. 10% BSA** Solution+: 30 ml (working dilution: 1:10)

Each monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The mouse anti-rat Ig antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed. All components of the Kit should be store undiluted at 4°C. Do not freeze.

Immunoglobulin (Ig), is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target, called an antigen. Each tip of the "Y" of an antibody contains a paratope (a structure analogous to a lock) that is specific for one particular epitope (similarly analogous to a key) on an antigen, allowing these two structures to bind together with precision. Using this binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize its target directly (for example, by blocking a part of a microbe that is essential for its invasion and survival). The production of antibodies is the main function of the humoral immune system.