Rat CRP reference serum (DAGA-688)

Alternative Names
Rat; CRP; Serum
Batch dependent - please inquire should you have specific requirements
0.1% Sodium Azide
Frozen -20°C
Antigen Description
C-reactive protein (CRP) is aprotein found in the blood, the levels of which rise in response toinflammation (i.e. C-reactive protein is an acute-phase protein). Itsphysiological role is to bind to phosphocholine expressed on the surface ofdead or dying cells (and some types of bacteria) in order to activate thecomplement system via the C1Q complex. CRP is synthesized by the liver inresponse to factors released by fat cells (adipocytes). It is a member of thepentraxin family of proteins. It is not related to C-peptide or protein C. C-reactiveprotein was the first pattern recognition receptor (PRR) to be identified.


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Highly specific nanobody against herbicide 2,4-dichlorophenoxyacetic acid for monitoring of its contamination in environmental water


Authors: Li, Zhen-Feng; Dong, Jie-Xian; Vasylieva, Natalia; Cui, Yong-Liang; Wan, De-Bin; Hua, Xiu-De; Huo, Jing-Qian; Yang, Dong-Chen; Gee, Shirley J.; Hammock, Bruce D.

2,4-dichlorophenoxyacetic acid (2,4-D), a widely used herbicide, is a small organic chemical pollutant in the environment. To develop a nanobody-based immunoassay for monitoring trace levels of 2,4-D, a step-wise strategy for the generation of nanobodies highly specific against this small chemical was employed. Firstly, we synthesized three novel haptens mimicking 2,4-D and assessed their influence on the sensitivity and specificity of the existing antibody-based assay. Polyclonal antibodies (pAb) from rabbits showed good sensitivity and moderate specificity for 2,4-D, pAb from llama based on selected haptens showed similar performance when compared to those from rabbits. Secondly, nanobodies derived from llama were generated for 2,4-D by an effective procedure, including serum monitoring and one-step library construction. One nanobody, NB3-9, exhibited good sensitivity against 2,4-D (IC50 = 29.2 ng/mL) had better specificity than the rabbit pAb#1518, with no cross-reactivities against the 2,4-D analogs tested. Thirdly, one-step fluorescent enzyme immunoassay (FLEIA) for 2,4-D based on a nanobody-alkaline phosphatase (AP) fusion was developed with IC50 of 1.9 ng/mL and a linear range of 0.4-8.6 ng/mL. Environmental water samples were analyzed by FLEIA and LC-MS/MS for comparison, and the results were consistent between both methods. Therefore, the proposed step-wise strategy from hapten design to nanobody-AP fusion production was successfully conducted, and the resulting nanobody based FLEIA was demonstrated as a convenient tool to monitor 2,4-D residuals in the environment. (C) 2020 Elsevier B.V. All rights reserved.

Fluorescent and electrochemical bimodal bioplatform for femtomolar detection of microRNAs in blood sera


Authors: Zayani, Riham; Rabti, Amal; Ben Aoun, Sami; Raouafi, Noureddine

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