Sample
Cell Culture Supernatants and Serum
Species Reactivity
Animal
Intended Use
The Rift Valley Fever Virus NP ELISA Development Kit contains the key components required for the quantitative analysis of NP (Rift Valley Fever Virus) concentrations in cell culture supernatants and serum within the range of 1 - 1000 ng/ml in a sandwich ELISA format. The components supplied in this kit are sufficient to assay NP in five 96-well ELISA plates.
Contents of Kit
1.Capture Antibody: 100 μl of 1 mg/ml anti-NP (Rift Valley Fever Virus) monoclonal antibody.
2.NP (Rift Valley Fever Virus) Standard: 50 μl of 50 μg/ml recombinant NP (Rift Valley Fever Virus).
3.Detection Antibody: 50 μl of anti-NP (Rift Valley Fever Virus) polyclonal antibody.
4.Anti-Rabbit-IgG-HRP Conjugate: 50 μl of HRP-conjugated anti-rabbit IgG.
Storage
Keep it at 4°C if used within a month. Forlong term storage, split it into small aliquots and keep at -80°C. Avoid repeated freezing and thawing. The product will be expired one year after receiving if stored properly. Non-hazardous. No MSDS required.
Detection Range
1 - 1000 ng/ml
Citations
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Elfadil, AA; Hasab-Allah, KA; et al. Factors associated with Rift Valley fever in south-west Saudi Arabia. REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES 25:1137-1145(2006).
BESSELAAR, TG; BLACKBURN, NK; et al. TOPOLOGICAL MAPPING OF ANTIGENIC SITES ON THE RIFT-VALLEY FEVER VIRUS ENVELOPE GLYCOPROTEINS USING MONOCLONAL-ANTIBODIES. ARCHIVES OF VIROLOGY 121:111-124(1991).