Rice Ragged Stunt Virus (RRSV) ELISA Kit

Sample

host plants

Intended Use

The test can be used to detect RRSV in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.

General Description

Rice ragged stunt virus (RRSV) is a plant pathogenic virus of the family Reoviridae.
RRSV, vectored by the brown planthopper (BPH) (Delphacidae: Nilaparvata lugens), causes ragged stunt disease of rice (Oryza spp., especially Oryza sativa). The virus, first described by Hibino, Ling and Shikata, is also less commonly known as rice infectious gall virus. RRSV has polyhedral particles about 50 nm in diameter possessing spikes (and perhaps a complete outer shell) to a diameter of 65 nm. The particles contain double-stranded RNA in eight segments. RRSV can only infect plants in the family Gramineae, i.e. grasses including rice. The virus can only be transmitted by BPH and is not mechanically transmitted to plants.

Standard Curve