General Notes
This product (Catalog No. CABT-BL3145) is a rabbit-derived polyclonal antibody raised against human RGL3, targeting the amino acid region 21–251 of the protein. It has been affinity purified by Protein A. RGL3 is a guanine nucleotide exchange factor. RGL3 mRNA is commonly detectable in several visceral organs (e.g. kidney, heart, liver, and lung) in the mouse and human. The RGL3 protein mainly localizes in the cytoplasm when expressed in fibroblasts. Yeast two-hybrid assay indicated that RGL3 could interact with Rap1, Rap2, H-Ras, N-Ras, and R-Ras but failed to interact efficiently with Ral and Rho (PMID: 17382517).
CABT-BL3145 is suitable for use in Western blot (WB) (1:500–1:2,000) application.
CABT-BL3145 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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