R848

Content
5 mg of lyophilized R848 Vaccine grade adjuvant
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Storage and Stability
R848 is shipped at room temperature and should be stored at 4°C or -20°C. Lyophilized product is stable 1 year when properly stored.
- Upon resuspension, prepare aliquots of R848 and store at -20°C for long term storage. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.

Description
R848 (resiquimod), a small molecular weight imidazoquinoline compound, is an immune response modifier with potent antiviral and antitumor activities. R848 is being evaluated as an adjuvant in FDA-approved clinical vaccine trials. R848 immune properties result from its ability to induce the production of pro-inflammatory cytokines through the activation of Toll-like receptor (TLR)-7 and TLR8. In vitro and in vivo studies have shown that R848 promotes the secretion of Th1 cytokines, including IFN-g, IFN-a, IL-12 and TNF-a. R848 is capable of skewing antibody responses toward a Th1 IgG2a response and away from a Th2 IgE response, a feature mediated in part by IFN-a and IL-12. Unlike most adjuvants, R848 can be administered by a different route than the antigen, suggesting that it does not produce a depot effect. Preclinical studies in mice have shown that R848 is able to promote adaptive immune responses to codelivered antigens and provide protection against live infection challenges.

Preparation of Sterile Stock Solution (1 mg/ml)
Add 5 ml endotoxin-free physiological water to the 5 mg R848 vial to obtain a solution at 1 mg/ml.
- Mix the solution by pipetting up and down.

Chemical Properties
CAS number: 144875-48-9
Formula: C17H22N4O2, HCl
Molecular weight: 350.8
Solubility: 1 mg/ml in physiological water

Vaccine Grade