Sample
plasma, cerebrospinal fluid
Detection Method
Sandwich ELISA
Intended Use
CD's L-PGDS EIA Kit is an immunometric (i.e., sandwich) EIA. The standard curve ranges from 0-100 ng/mL, with a limit of detection of 6 ng/mL. Inter- and intra-assay CV's of less than 15% may be achieved at most concentrations. L-PGDS is present in a variety of body fluids including cerebrospinal fluid, seminal fluid, and plasma. This assay has been validated using cerebrospinal fluid which contains approximately 12-30 μg/ml of L-PGDS.
Contents of Kit
1. Anti-L-PGDS (human) EIA Strip Plate, 1 plate
2. L-PGDS (human) EIA Biotinylated Detection Antibody, 1 vial/100 dtn
3. L-PGDS (human) EIA Standard, 3 vials
4. EIA Buffer Concentrate (10x), 2 vials/10 mL
5. Wash Buffer Concentrate (400X), 1 vial/5 mL
6. Polysorbate 20, 1 vial/3 mL
7. 96-Well Cover Sheet, 1 cover
8. Ellman's Reagent, 3 vials/100 dtn
9. Non-specific Mouse Serum, 1 vial/100 dtn
10. Avidin-Acetylcholinesterase Conjugate, 1 vial/100 dtn
Storage
This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box. For more detailed information, please download the following document on our website.
Citations
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