10 mg of lyophilized Poly(I:C) HMW Vaccine grade adjuvant
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
Note: This product is dispensed by weight of dry material. This weight includes polymer, residual salt and residual water. Content of polymer may vary from lot to lot.
Storage and Stability
Poly(I:C) HMW is shipped at room temperature and should be stored at 4°C. Lyophilized product is stable 1 year at 4°C when properly stored.
- Upon resuspension, prepare aliquots of Poly(I:C) and store at -20°C for long term storage. Store at 4°C for short term storage.
- Resuspended product is stable 1 month at 4°C and 1 year at -20°C. Avoid repeated freeze-thaw cycles.
CAS number: 31852-29-6
Average size: 1.5 - 8 kb
solubility: 5 mg/ml in water
Working concentration: 10-100 μg/mouse
Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection. Poly(I:C) can activate the immune response through two distinct pathogen recognition receptors (PRRs). Endosomal poly(I:C) activates TLR3 while cytosolic poly(I:C) activates RIG-I/MDA-5. Triggering the TLR3 pathway induces IL-12 and type I IFNs production, and improves MHC class II expression and cross-presentation of antigen. Stimulation of MDA-5 enhances the production of type I IFNs that play a critical role in enhancing T and B cell immunity. Poly(I:C) promotes Th1 (cellular) biased immunity through its induction of IL-12 and type I IFNs.
Poly(I:C) has been tested as an adjuvant in numerous animal models. Promising results have been obtained using poly(I:C) as an adjuvant in flu vaccine delivered intranasally to mice. Poly(I:C) has also been shown to enhance the efficacy of peptide-based cancer vaccines by promoting tumor specific T cell responses in mice. Immunization of mice with poly(I:C) resulted in a strong Th1 response and high levels of serum type I IFN.
Preparation of Sterile Stock Solution (1 mg/ml)
Add 10 ml of the endotoxin-free physiological water provided to the 10 mg Poly(I:C) vial.
- Mix the solution by pipetting up and down.
- Heat the mixture for 10 minutes at 65 - 70°C. Allow the solution to cool for 1 hour at room temperature to ensure proper annealing.