Vaccine Grade

Poly(I:C) HMW

  • Product Name
  • Poly(I:C) HMW / Polyinosinic-polycytidylic acid
  • Cat. No.
  • ADJU-023CL
  • Content
  • 10 mg of lyophilized Poly(I:C) HMW Vaccine grade adjuvant
    10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
    Note: This product is dispensed by weight of dry material. This weight includes polymer, residual salt and residual water. Content of polymer may vary from lot to lot.
  • Storage and Stability
  • Poly(I:C) HMW is shipped at room temperature and should be stored at 4°C. Lyophilized product is stable 1 year at 4°C when properly stored.
    - Upon resuspension, prepare aliquots of Poly(I:C) and store at -20°C for long term storage. Store at 4°C for short term storage.
    - Resuspended product is stable 1 month at 4°C and 1 year at -20°C. Avoid repeated freeze-thaw cycles.
  • Chemical Properties

  • CAS number: 31852-29-6
    Average size: 1.5 - 8 kb
    solubility: 5 mg/ml in water
    Working concentration: 10-100 μg/mouse
  • Description

  • Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection. Poly(I:C) can activate the immune response through two distinct pathogen recognition receptors (PRRs). Endosomal poly(I:C) activates TLR3 while cytosolic poly(I:C) activates RIG-I/MDA-5. Triggering the TLR3 pathway induces IL-12 and type I IFNs production, and improves MHC class II expression and cross-presentation of antigen. Stimulation of MDA-5 enhances the production of type I IFNs that play a critical role in enhancing T and B cell immunity. Poly(I:C) promotes Th1 (cellular) biased immunity through its induction of IL-12 and type I IFNs.
    Poly(I:C) has been tested as an adjuvant in numerous animal models. Promising results have been obtained using poly(I:C) as an adjuvant in flu vaccine delivered intranasally to mice. Poly(I:C) has also been shown to enhance the efficacy of peptide-based cancer vaccines by promoting tumor specific T cell responses in mice. Immunization of mice with poly(I:C) resulted in a strong Th1 response and high levels of serum type I IFN.
  • Preparation of Sterile Stock Solution (1 mg/ml)
  • Add 10 ml of the endotoxin-free physiological water provided to the 10 mg Poly(I:C) vial.
    - Mix the solution by pipetting up and down.
    - Heat the mixture for 10 minutes at 65 - 70°C. Allow the solution to cool for 1 hour at room temperature to ensure proper annealing.

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