Human Anti-Polio Virus 1-3 IgG ELISA Kit

Sample

Serum, Plasma or other Biological Fluids

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Human Anti-Polio Virus 1-3 IgG ELISA Kit detects and quantifies polio type 1, 2 & 3 IgG in human serum or plasma of exposed or immunized animals. This immunoassay is suitable for:
Determining immune status relative to non-immune controls;
Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing;
Qualifying and/or standardizing vaccine batches and protocols.
This kit is for research use only (RUO), not for diagnosis or therapeutic purposes.

Contents of Kit

1.Polio 1-3 Antigens Coated Strip Plate, 8-well strips (12). Coated with polio 1-3 capsid proteins; postcoated with stabilizers.
2.Anti- Polio IgG Calibrators. Four (4) vials, each containing anti- polio antibodies; in buffer with antimicrobial.
Anti-Polio IgG Calibrator 3 U/ml, 1.0 ml
Anti-Polio IgG Calibrator 10 U/ml, 1.0 ml
Anti-Polio IgG Calibrator 30 U/ml, 1.0 ml
Anti-Polio IgG Calibrator 90 U/ml, 1.0 ml
3.Anti-Polio IgG Positive Control, 1.0 ml. Anti-polio diluted in buffer with protein, detergents and antimicrobial. [Value range on label]
4.Anti-Human IgG HRP Conjugate (100X), 0.15 ml
5.Sample Diluent (20x), 10 ml
6.Low NSB Sample Diluent, 30 ml.Reduces non-specific binding. Not for HRP Conjugate dilution. Buffer with protein, detergents and antimicrobial. Use as is for sample dilution. See Assay Design.
7.Wash Solution Concentrate (100X), 10 ml
8.TMB Substrate, 12 ml.Chromogenic substrate for HRP containing TMB and peroxide.
9.Stop Solution, 12 ml. Dilute sulfuric acid.
10.Product Manual, 1 ea

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label. Stabilities of the working solutions are indicated under Reagent Preparation.

General Description

Poliomyelitis, often called polio or infantile paralysis, is an acute viral infectious disease caused by any of the three serotypes of polioviruses (PV1, PV-2, and PV-3). Type 1 (Brunhilde): often with severe symptoms, Type 2 (Lansing): with milder symptoms, Type 3 (Leon): rare, but with severe symptoms. Antibodies to poliovirus can be diagnostic, and are generally detected in the blood of infected patients early in the course of infection. Two types of vaccines are used throughout the world to combat polio: 1) Salk vaccine, or inactivated poliovirus vaccine (IPV), is based on three wild, virulent reference strains, Mahoney (type 1), MEF-1 (type 2), and Saukett (type 3) polio viruses, grown in a type of monkey kidney tissue culture (Vero cell line), which are then inactivated with formalin; 2) Oral polio vaccine (OPV or Sabin's vaccine) is a live-attenuated vaccine, produced by the passage of the virus through non-human cells at a sub-physiological temperature, which produces spontaneous mutations in the viral genome.