Sample
cell culture supernate, serum, plasma
Intended Use
For the development of sandwich ELISAs to measure natural and recombinant human Parkinson Disease Gene7 (Park7). The Reagent Diluent recommended may be suitable for most cell culture supernate, serum, and plasma samples. The Reagent Diluent selected for use can alter the performance of an immunoassay. Reagent Diluent optimization for samples with complex matrices such as serum and plasma, may improve their performance in this assay.
This kit contains sufficient materials to run ELISAs on at least fifteen 96 well plates, provided the following conditions are met:
- The reagents are prepared as described in this package insert.
- The assay is run as described in the General ELISA Protocol.
- The recommended microplates, buffers, diluents, substrates, and solutions are used.
Contents of Kit
Human Park7 Capture Antibody: 1 vial
Human Park7 Detection Antibody: 1 vial
Human Park7 Standard: 1 vial
Streptavidin-HRP: 1 vial
Storage
Store the unopened kit at 2-8°C. Do not use past kit expiration date.
Citations
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Q & A
Q: If the antibodies in this kit are polyclonal or monoclonal?
A: The antibody is a goat polyclonal antibody
Customer Reviews
Curi, NHD; Miranda, I; et al. Serologic evidence of Leishmania infection in free-ranging wild and domestic canids around a Brazilian National Park. MEMORIAS DO INSTITUTO OSWALDO CRUZ 101:99-101(2006).
Yang, L; Lee, O; et al. Human acyl-coenzyme A : cholesterol acyltransferase 1 (acat1) sequences located in two different chromosomes (7 and 1) are required to produce a novel ACAT1 isoenzyme with additional sequence at the N terminus. JOURNAL OF BIOLOGICAL CHEMISTRY 279:46253-46262(2004).
COA
Certificate of Analysis
